Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE035GU)

• DME Name: D-alanylalanine synthetase (ddl)
• Synonyms: Alanylalanine synthetase; D-D-Ala-D-Ala ligase; D-D-alanine--D-alanine ligase; DW856_04795; BHW46_13680
• Gene Name: ddl
• UniProt ID: A0A1Q6S944_9FIRM
• INTEDE ID: DME1337
• EC Number:
  EC: 6.3.2.4
  Ligase
  Carbon-nitrogen ligase
  Peptide synthase
  EC: 6.3.2.4
• Lineage:
  Species: Roseburia intestinalis
  Kingdom: Bacteria
  Phylum: Firmicutes
  Class: Clostridia
  Order: Clostridiales
  Family: Lachnospiraceae
  Genus: Roseburia
  Species: Roseburia intestinalis
  Subspecies: Roseburia intestinalis XB6B3
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MKIVVLAGGLSTERDVSFKTGDMVAKALRENGHQVILLDVFMGYSDKKEDLTGIFDRSEE
  VSVKVSGIPETAPDLAKVKASRKDQSDNFFGPNVIELCQMSDIVFMALHGENGENGKIQA
  AFDLFGIRYTGTGYLSSALAMDKEMSKKLFLSAGIPAPKGASMKKADCSDDITKLGIQFP
  CVVKPCCGGSSIGVSIAHNAEEYKAALDEAFRWEENLIVEEYVKGREFSVGVIEGKALPI
  IEIAPVQGFYDYKNKYKAGSAVETCPAELSEAVTEKMQHYAEQVAQVLGLDTYSRTDFLL
  DENENIYCLEANTLPGMTPTSLLPQEAQVVGVNFNELCEKLIQISLDKYEK
• Function: This enzyme is involved with UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase (EC 6.3.2.7) or UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---2,6-diaminopimelate ligase (EC 6.3.2.13), UDP-N-acetylmuramate---L-alanine ligase (EC 6.3.2.8), UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase (EC 6.3.2.9) and UDP-N-acetylmuramoyl-tripeptide---D-alanyl-D-alanine ligase (EC 6.3.2.10) in the synthesis of a cell-wall peptide.

Molecular Interaction Atlas (MIA) of This DME

1 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Vancomycin	DM3JFIH	Bacteremia	1A73	Approved	[1]

References

• [1] vanG element insertions within a conserved chromosomal site conferring vancomycin resistance to Streptococcus agalactiae and Streptococcus anginosus. mBio. 2014 Jul 22;5(4):e01386-14.