Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE6ZDK4)

DME Name Cytochrome P450 125A3 (cyp125)
Synonyms
Cytochrome P450 family 125 subfamily A member 3; Cholest-4-en-3-one 26-monooxygenase 125A3; Cholest-4-en-3-one C26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming] 125A3; Cholesterol C26-monooxygenase 125A3; Cholesterol C26-monooxygenase [(25S)-3beta-hydroxycholest-5-en-26-oate forming] 125A3; MSMEG_5995; Steroid C26-monooxygenase 125A3; Steroid C27-monooxygenase 125A3; cyp125A3
Gene Name cyp125
UniProt ID
CP125_MYCS2
INTEDE ID
DME1549
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Gene ID
4531666
EC Number EC: 1.14.15.29
Oxidoreductase
Oxygen paired donor oxidoreductase
Iron-sulfur protein donor oxidoreductase
EC: 1.14.15.29
Lineage Species: Mycolicibacterium smegmatis
Kingdom: Bacteria
Phylum: Actinobacteria
Class: Actinobacteria
Order: Corynebacteriales
Family: Mycobacteriaceae
Genus: Mycolicibacterium
Species: Mycolicibacterium smegmatis
Tissue Distribution Primarily distributed in human lung.
Sequence
MPTPNIPSDFDFLDATLNLERLPVEELAELRKSEPIHWVDVPGGTGGFGDKGYWLVTKHA
DVKEVSRRSDVFGSSPDGAIPVWPQDMTREAVDLQRAVLLNMDAPQHTRLRKIISRGFTP
RAIGRLEDELRSRAQKIAQTAAAQGAGDFVEQVSCELPLQAIAELLGVPQDDRDKLFRWS
NEMTAGEDPEYADVDPAMSSFELISYAMKMAEERAVNPTEDIVTKLIEADIDGEKLSDDE
FGFFVVMLAVAGNETTRNSITHGMIAFAQNPDQWELYKKERPETAADEIVRWATPVSAFQ
RTALEDVELGGVQIKKGQRVVMSYRSANFDEEVFEDPHTFNILRSPNPHVGFGGTGAHYC
IGANLARMTINLIFNAIADNMPDLKPIGAPERLKSGWLNGIKHWQVDYTGAGKASVSGAP
GTCPVAH
Function
This enzyme is a P-450 heme-thiolate protein found in several bacterial pathogens, and it is involved in degradation of the host's cholesterol. The enzyme catalyses the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol . The products are exclusively in the (25S) configuration. And it also accepts cholesterol as a substrate.
KEGG Pathway
Microbial metabolism in diverse environments (msg01120 )
Steroid degradation (msg00984 )

Molecular Interaction Atlas (MIA) of This DME

Molecular Interaction Atlas (MIA) Jump to Detail Molecular Interaction Atlas of This DME
1 Clinical Trial Drug(s) Metabolized by This DME
Drug Name Drug ID Indication ICD 11 Highest Status REF
ANW-32821 DMMJOZD N. A. N. A. Phase 2 [1]

References

1 Cholesterol ester oxidation by mycobacterial cytochrome P450. J Biol Chem. 2014 Oct 31;289(44):30417-25.