Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEA742Z)

DME Name Cysteinyl-conjugate N-acetyltransferase (NAT8)
Synonyms Acetyltransferase 2; Camello-like protein 1; GLA; N-acetyltransferase 8; ATase2; CCNAT; CML1; NAT8; TSC501
Gene Name NAT8
UniProt ID
NAT8_HUMAN
INTEDE ID
DME0336
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Gene ID
9027
EC Number EC: 2.3.1.80
Transferase
Acyltransferase
Acyltransferase
EC: 2.3.1.80
Lineage Species: Homo sapiens
Kingdom: Metazoa
Phylum: Chordata
Class: Mammalia
Order: Primates
Family: Hominidae
Genus: Homo
Species: Homo sapiens
Sequence
MAPCHIRKYQESDRQWVVGLLSRGMAEHAPATFRQLLKLPRTLILLLGGPLALLLVSGSW
LLALVFSISLFPALWFLAKKPWTEYVDMTLCTDMSDITKSYLSERGSCFWVAESEEKVVG
MVGALPVDDPTLREKRLQLFHLFVDSEHRRQGIAKALVRTVLQFARDQGYSEVILDTGTI
QLSAMALYQSMGFKKTGQSFFCVWARLVALHTVHFIYHLPSSKVGSL
Function
This enzyme acetylates the free alpha-amino group of cysteine S-conjugates to form mercapturic acids. This is the final step in a major route for detoxification of a wide variety of reactive electrophiles which starts with their incorporation into glutathione S-conjugates. The glutathione S-conjugates are then further processed into cysteine S-conjugates and finally mercapturic acids which are water soluble and can be readily excreted in urine or bile. Alternatively, it may have a lysine N-acetyltransferase activity catalyzing peptidyl-lysine N6-acetylation of various proteins.
KEGG Pathway
Glutathione metabolism (hsa00480 )
Metabolic pathways (hsa01100 )
Reactome Pathway
Amyloid fiber formation (R-HSA-977225 )

Molecular Interaction Atlas (MIA) of This DME

Molecular Interaction Atlas (MIA) Jump to Detail Molecular Interaction Atlas of This DME
1 Investigative Drug(s) Metabolized by This DME
Drug Name Drug ID Indication ICD 11 Highest Status REF
acetyl CoA DMH65Q8 Discovery agent N.A. Investigative [1]
Experimental Enzyme Kinetic Data of Drugs
Drug Name Indication Highest Status Kinetic Data REF
acetyl CoA Discovery agent [N.A.] Investigative Km = 0.023 microM [1]

References

1 Molecular identification of NAT8 as the enzyme that acetylates cysteine S-conjugates to mercapturic acids. J Biol Chem. 2010 Jun 11;285(24):18888-98.