Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEB90AL)

• DME Name: Carbapenemase (cphA)
• Synonyms: Versatile beta-lactamase; Versatile hydrolytic beta-lactamase; Beta-lactamase II; Carbapenem-hydrolyzing metallo-beta-lactamase; Metallo-beta-lactamase type 2b; Metallo-beta-lactamase type IIb; cphA
• Gene Name: cphA
• UniProt ID: BLAB_AERHY
• INTEDE ID: DME1731
• EC Number:
  EC: 3.5.2.6
  Hydrolases
  Carbon-nitrogen hydrolase
  Cyclic amide hydrolase
  EC: 3.5.2.6
• Lineage:
  Species: Aeromonas hydrophila
  Kingdom: Bacteria
  Phylum: Proteobacteria
  Class: Gammaproteobacteria
  Order: Aeromonadales
  Family: Aeromonadaceae
  Genus: Aeromonas
  Species: Aeromonas hydrophila
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MMKGWMKCGLAGAVVLMASFWGGSVRAAGMSLTQVSGPVYVVEDNYYVQENSMVYFGAKG
  VTVVGATWTPDTARELHKLIKRVSRKPVLEVINTNYHTDRAGGNAYWKSIGAKVVSTRQT
  RDLMKSDWAEIVAFTRKGLPEYPDLPLVLPNVVHDGDFTLQEGKVRAFYAGPAHTPDGIF
  VYFPDEQVLYGNCILKEKLGNLSFADVKAYPQTLERLKAMKLPIKTVIGGHDSPLHGPEL
  IDHYEALIKAAPQS
• Function: This enzyme confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. It is able to hydrolyze penicillin and imipenem, but is much less active against cephalothin, cefotaxime, meropenem and ceftazidime.

Molecular Interaction Atlas (MIA) of This DME

2 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Imipenem	DMI9FBP	Bacteremia	1A73	Approved	[1], [2]
Meropenem	DM62UHC	Bacterial infection	1A00-1C4Z	Approved	[1], [2]

References

• [1] A carbapenem-resistant clinical isolate of Aeromonas hydrophila in Japan harbouring an acquired gene encoding GES-24 beta-lactamase. J Med Microbiol. 2018 Nov;67(11):1535-1537.
• [2] Chronic colitis after Aeromonas infection. Gut. 1989 May;30(5):686-90. Case Reports