Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEBI82Z)

• DME Name: Alpha-L-rhamnosidase (lrA)
• Synonyms: Alpha-L-rhamnosidase T; Alpha-L-rhamnosidase N; Alpha-L-rhamnoside rhamnohydrolase; B5790_0114
• Gene Name: lrA
• UniProt ID: A0A1V8Q7P3_9BIF
• INTEDE ID: DME1201
• EC Number:
  EC: 3.2.1.40
  Hydrolases
  Glycosylase
  O/S-glycosyl compound glycosidase
  EC: 3.2.1.40
• Lineage:
  Species: Bifidobacterium dentium
  Kingdom: Bacteria
  Phylum: Actinobacteria
  Class: Actinobacteria
  Order: Bifidobacteriales
  Family: Bifidobacteriaceae
  Genus: Bifidobacterium
  Species: Bifidobacterium dentium
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MDTQIIKPIAVGDLHITRFTIEHFPGDALGIPTATPRMNWTYSRTVPDGSQILLKISRRI
  PGAQAREEYTYLPPEDNVLVPWQFTPLASREEVFATVQLVSAAHKPLSDPSATLHFEAGL
  MQEYEHVADFVGPSWAEPETDHRHLPLVRTEIAIKAKPKRARLYLSALGLVEAEINGVKI
  GNDALIPGWTNYEQRVQCWTYDVTDELNEGANAMGFWLGDGWYRGRLGFDGGYVNYYGDR
  LGVFAQLEIEYEDGGIEHVYSNAWDRQWKASLGPIVCSDLCEGERYDARLEQPGWSEPGF
  DDSNWKPVSEIFYDPAKIENPDTSPVRAHEINEPVSIARTGDTPDGRGIWLVDFGQNCTQ
  RIRLRIAGLEAGQSVELHHVEVLEPNGEIATRTLRRGQQHDIYTSNGTDAWWEPRFAMHG
  FRYAQIEGVVGELTVADIECHVYHSVMDRAGELETSNPLLNRLHANALWSMRSNFVSVPQ
  DCPQRDERMGWTGDICLFAPTAAYLYDVYGFLKSWLKDVRADQVKWGTVPFYVPFIPLGG
  WAHPQAISTWGDSAVEVPWVLYMESGDPQILADSYELSRDWIDEVAGYLSADGVWDRRPD
  YVLGQLGDWLDPTAPPDDPTQAMTEKELVATAFFARSCAQAEQIAQILGKSEDVVRFGDL
  RKHVTSGFLGRFTNLDGTMTSDTQCAYALAIAFGLLDGEPVRRVKAGNRLAKLVRESGGK
  VSTGFAGTPFVLPALTMTGHNEEAYGLLLSEECPSWLYQVKMGATTTWERWDSMRPDGSL
  NPGGMTSFNHYALGSVAEWMHAHIGGLEAIEAGWKRFRVAPVIGGGIDHASTSHVTPYGK
  ASVAWKTSETGVELDVTVPIGATAVVELPDHEPTELIGGTHHLVVAL
• Function: This enzyme utilizes an inverting mechanism of hydrolysis, releasing beta-L-rhamnose. Substrates include naringin, rutin, quercitrin, hesperidin, dioscin, terpenyl glycosides and many other natural glycosides containing terminal alpha-L-rhamnose.

Molecular Interaction Atlas (MIA) of This DME

1 Preclinical Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
HY-N2258	DML4IAX	N. A.	N. A.	Preclinical	[1]

4 Investigative Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Ginsenoside Re	DM46FVD	N. A.	N. A.	Investigative	[1]
naringin	DM4DG1Y	Discovery agent	N.A.	Investigative	[1]
PNA-l-rhamnopyranoside	DMZF7RQ	N. A.	N. A.	Investigative	[1]
Rutin	DMEHRAJ	Discovery agent	N.A.	Investigative	[1]

Experimental Enzyme Kinetic Data of Drugs

Drug Name	Indication	Highest Status	Kinetic Data	REF
Rutin	Discovery agent [N.A.]	Investigative	Km = 2190 microM	[1]

References

• [1] Metabolism of rutin and poncirin by human intestinal microbiota and cloning of their metabolizing alpha-L-rhamnosidase from Bifidobacterium dentium. J Microbiol Biotechnol. 2015 Jan;25(1):18-25.