Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEH73ZC)

• DME Name: VanC2 ligase (vanC)
• Synonyms: Vancomycin C-type resistance protein VanC; VanC ligase; vanC; vanC-2; vanC2; NCTC12361_02496
• Gene Name: vanC-2
• UniProt ID: Q47720_ENTCA
• INTEDE ID: DME1297
• EC Number:
  EC: 6.3.2.4
  Ligase
  Carbon-nitrogen ligase
  Peptide synthase
  EC: 6.3.2.4
• Lineage:
  Species: Enterococcus casseliflavus
  Kingdom: Bacteria
  Phylum: Firmicutes
  Class: Bacilli
  Order: Lactobacillales
  Family: Enterococcaceae
  Genus: Enterococcus
  Species: Enterococcus casseliflavus
  Subspecies: Enterococcus casseliflavus ATCC 25788
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MKKIAIIFGGNSPEYTVSLASATSAIEALQSSPYDYDLSLIGIAPDAMDWYLYTGELENI
  RQDTWLLDTKHKQKIQPLFEGNGFWLSEEQQTLVPDVLFPIMHGKYGEDGSIQGLFELMK
  LPYVGCGVAGSALCMNKWLLHQAAAAIGVQSAPTILLTNQANQQEQIEAFIQTHGFPVFF
  KPNEAGSSKGITKVTCVEEIASALKEAFTYCSAVLLQKNIAGVEIGCGILGNDSLTVGAC
  DAISLVDGFFDFEEKYQLISAKITVPAPLPETIETKVKEQAQLLYRSLGLKGLARIDFFV
  TERGELYLNEINTMPGFTSHSRYPAMMAAVGLSYQELLQKLLVLAKEEVK
• Function: This enzyme is involved with UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---L-lysine ligase (EC 6.3.2.7) or UDP-N-acetylmuramoyl-L-alanyl-D-glutamate---2,6-diaminopimelate ligase (EC 6.3.2.13), UDP-N-acetylmuramate---L-alanine ligase (EC 6.3.2.8), UDP-N-acetylmuramoyl-L-alanine---D-glutamate ligase (EC 6.3.2.9) and UDP-N-acetylmuramoyl-tripeptide---D-alanyl-D-alanine ligase (EC 6.3.2.10) in the synthesis of a cell-wall peptide.

Molecular Interaction Atlas (MIA) of This DME

1 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Vancomycin	DM3JFIH	Bacteremia	1A73	Approved	[1]

References

• [1] Bacterial resistance to vancomycin: overproduction, purification, and characterization of VanC2 from Enterococcus casseliflavus as a D-Ala-D-Ser ligase. Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10040-4.