Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DELIXR8)

• DME Name: Cytochrome P450 125A1 (cyp125)
• Synonyms: Cytochrome P450 family 125 subfamily A member 1; Cholest-4-en-3-one 26-monooxygenase 125A1; Cholest-4-en-3-one C26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming] 125A1; Cholesterol C26-monooxygenase 125A1; Cholesterol C26-monooxygenase [(25S)-3beta-hydroxycholest-5-en-26-oate forming] 125A1; MTCY03C7.11; Rv3545c; Steroid C26-monooxygenase 125A1; Steroid C27-monooxygenase 125A1; cyp125A1
• Gene Name: cyp125
• UniProt ID: CP125_MYCTU
• INTEDE ID: DME1551
• Gene ID: 887782
• EC Number:
  EC: 1.14.15.29
  Oxidoreductase
  Oxygen paired donor oxidoreductase
  Iron-sulfur protein donor oxidoreductase
  EC: 1.14.15.29
• Lineage:
  Species: Mycobacterium tuberculosis
  Kingdom: Bacteria
  Phylum: Actinobacteria
  Class: Actinobacteria
  Order: Corynebacteriales
  Family: Mycobacteriaceae
  Genus: Mycobacterium
  Species: Mycobacterium tuberculosis
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MSWNHQSVEIAVRRTTVPSPNLPPGFDFTDPAIYAERLPVAEFAELRSAAPIWWNGQDPG
  KGGGFHDGGFWAITKLNDVKEISRHSDVFSSYENGVIPRFKNDIAREDIEVQRFVMLNMD
  APHHTRLRKIISRGFTPRAVGRLHDELQERAQKIAAEAAAAGSGDFVEQVSCELPLQAIA
  GLLGVPQEDRGKLFHWSNEMTGNEDPEYAHIDPKASSAELIGYAMKMAEEKAKNPADDIV
  TQLIQADIDGEKLSDDEFGFFVVMLAVAGNETTRNSITQGMMAFAEHPDQWELYKKVRPE
  TAADEIVRWATPVTAFQRTALRDYELSGVQIKKGQRVVMFYRSANFDEEVFQDPFTFNIL
  RNPNPHVGFGGTGAHYCIGANLARMTINLIFNAVADHMPDLKPISAPERLRSGWLNGIKH
  WQVDYTGRCPVAH
• Function: This enzyme is a P-450 heme-thiolate protein found in several bacterial pathogens, and it is involved in degradation of the host's cholesterol. The enzyme catalyses the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol . The products are exclusively in the (25S) configuration. And it also accepts cholesterol as a substrate.
• KEGG Pathway:
  Microbial metabolism in diverse environments (mtu01120)
  Steroid degradation (mtu00984)

Molecular Interaction Atlas (MIA) of This DME

1 Clinical Trial Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
ANW-32821	DMMJOZD	N. A.	N. A.	Phase 2	[1]

References

• [1] Function, essentiality, and expression of cytochrome P450 enzymes and their cognate redox partners in Mycobacterium tuberculosis: are they drug targets? Appl Microbiol Biotechnol. 2019 May;103(9):3597-3614.