Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEPUK4S)

DME Name Cytochrome P450 142A2 (cyp142)
Synonyms
Cytochrome P450 family 142 subfamily A member 2; Cholest-4-en-3-one C26-monooxygenase 142A2; Cholest-4-en-3-one C26-monooxygenase [(25R)-3-oxocholest-4-en-26-oate forming] 142A2; Cholesterol C26-monooxygenase 142A2; Cholesterol C26-monooxygenase [(25R)-3beta-hydroxycholest-5-en-26-oate forming] 142A2; MSMEG_5918; Steroid C26-monooxygenase 142A2; Steroid C27-monooxygenase 142A2; cyp142A2
Gene Name cyp142
UniProt ID
CP142_MYCS2
INTEDE ID
DME1550
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Gene ID
4533135
EC Number EC: 1.14.15.28
Oxidoreductase
Oxygen paired donor oxidoreductase
Iron-sulfur protein donor oxidoreductase
EC: 1.14.15.28
Lineage Species: Mycolicibacterium smegmatis
Kingdom: Bacteria
Phylum: Actinobacteria
Class: Actinobacteria
Order: Corynebacteriales
Family: Mycobacteriaceae
Genus: Mycolicibacterium
Species: Mycolicibacterium smegmatis
Tissue Distribution Primarily distributed in human lung.
Sequence
MTQMLTRPDVDLVNGMFYADGGAREAYRWMRANEPVFRDRNGLAAATTYQAVLDAERNPE
LFSSTGGIRPDQPGMPYMIDMDDPQHLLRRKLVNAGFTRKRVMDKVDSIGRLCDTLIDAV
CERGECDFVRDIAAPLPMAVIGDMLGVLPTERDMLLKWSDDLVCGLSSHVDEAAIQKLMD
TFAAYTEFTKDVITKRRAEPTDDLFSVLVNSEVEGQRMSDDEIVFETLLILIGGDETTRH
TLSGGTEQLLRHRDQWDALVADVDLLPGAIEEMLRWTSPVKNMCRTLTADTVFHGTELRA
GEKIMLMFESANFDESVFGDPDNFRIDRNPNSHVAFGFGTHFCLGNQLARLELRLMTERV
LRRLPDLRLADDAPVPLRPANFVSGPESMPVVFTPSAPVLA
Function
This enzyme is a P-450 heme-thiolate protein, and it is involved in the utilization of cholesterol as the sole carbon and energy source by degrading the side chain. Primarily catalyzes the sequential oxidation of the terminal methyl of cholest-4-en-3-one into (25R)-26-hydroxycholest-4-en-3-one (alcohol), (25R)-26-oxocholest-4-en-3-one (aldehyde), to finally yield the carboxylic acid (25R)-3-oxocholest-4-en-26-oate. Also able to sequentially oxidize cholesterol itself, not only cholest-4-en-3-one.
KEGG Pathway
Microbial metabolism in diverse environments (msb01120 )
Steroid degradation (msb00984 )

Molecular Interaction Atlas (MIA) of This DME

Molecular Interaction Atlas (MIA) Jump to Detail Molecular Interaction Atlas of This DME
1 Clinical Trial Drug(s) Metabolized by This DME
Drug Name Drug ID Indication ICD 11 Highest Status REF
ANW-32821 DMMJOZD N. A. N. A. Phase 2 [1]

References

1 Cholesterol ester oxidation by mycobacterial cytochrome P450. J Biol Chem. 2014 Oct 31;289(44):30417-25.