Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DER7KFQ)

DME Name VanB ligase (vanB)
Synonyms Vancomycin B-type resistance protein VanB; D-alanylalanine synthetase VanB; D-alanine--D-alanine ligase VanB; D-Ala-D-Ala ligase VanB; vanB; vanB2; EF_2294
Gene Name vanB
UniProt ID
VANB_ENTFA
INTEDE ID
DME1292
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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Gene ID
1201166
EC Number EC: 6.3.2.4
Ligase
Carbon-nitrogen ligase
Peptide synthase
EC: 6.3.2.4
Lineage Species: Enterococcus faecalis
Kingdom: Bacteria
Phylum: Firmicutes
Class: Bacilli
Order: Lactobacillales
Family: Enterococcaceae
Genus: Enterococcus
Species: Enterococcus faecalis
Tissue Distribution Primarily distributed in human gut.
Sequence
MNKIKVAIIFGGCSEEHDVSVKSAIEIAANINTEKFDPHYIGITKNGVWKLCKKPCTEWE
ADSLPAIFSPDRKTHGLLVMKEREYETRRIDVAFPVLHGKCGEDGAIQGLFELSGIPYVG
CDIQSSAACMDKSLAYILTKNAGIAVPEFQMIEKGDKPEARTLTYPVFVKPARSGSSFGV
TKVNSTEELNAAIEAAGQYDGKILIEQAISGCEVGCAVMGNEDDLIVGEVDQIRLSHGIF
RIHQENEPEKGSENAMIIVPADIPVEERNRVQETAKKVYRVLGCRGLARVDLFLQEDGGI
VLNEVNTLPGFTSYSRYPRMAAAAGITLPALIDSLITLAIER
Function
This enzyme is required for high-level resistance to glycopeptides antibiotics. D-Ala--D-Ala ligase of altered specificity catalyzes ester bond formation between D-Ala and various D-hydroxy acids and produce a peptidoglycan which does not terminate in D-alanine but in D-lactate, thus preventing vancomycin binding.
KEGG Pathway
Two-component system (efa02020 )
Vancomycin resistance (efa01502 )

Molecular Interaction Atlas (MIA) of This DME

Molecular Interaction Atlas (MIA) Jump to Detail Molecular Interaction Atlas of This DME
1 Approved Drug(s) Metabolized by This DME
Drug Name Drug ID Indication ICD 11 Highest Status REF
Vancomycin DM3JFIH Bacteremia 1A73 Approved [1]

References

1 Genetics and mechanisms of glycopeptide resistance in enterococci. Antimicrob Agents Chemother. 1993 Aug;37(8):1563-71.