Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DETN1O0)

• DME Name: Uridine-cytidine kinase 2 (UCK2)
• Synonyms: Cytidine monophosphokinase 2; Uridine monophosphokinase 2; Testis-specific protein TSA903; UCK 2; UCK2
• Gene Name: UCK2
• UniProt ID: UCK2_HUMAN
• INTEDE ID: DME0480
• Gene ID: 7371
• EC Number:
  EC: 2.7.1.48
  Transferase
  Kinase
  Phosphotransferase
  EC: 2.7.1.48
• Lineage:
  Species: Homo sapiens
  Kingdom: Metazoa
  Phylum: Chordata
  Class: Mammalia
  Order: Primates
  Family: Hominidae
  Genus: Homo
  Species: Homo sapiens
• Sequence:
  MAGDSEQTLQNHQQPNGGEPFLIGVSGGTASGKSSVCAKIVQLLGQNEVDYRQKQVVILS
  QDSFYRVLTSEQKAKALKGQFNFDHPDAFDNELILKTLKEITEGKTVQIPVYDFVSHSRK
  EETVTVYPADVVLFEGILAFYSQEVRDLFQMKLFVDTDADTRLSRRVLRDISERGRDLEQ
  ILSQYITFVKPAFEEFCLPTKKYADVIIPRGADNLVAINLIVQHIQDILNGGPSKRQTNG
  CLNGYTPSRKRQASESSSRPH
• Function: This enzyme phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate but does not phosphorylate deoxyribonucleosides or purine ribonucleosides. It can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)- acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.
• KEGG Pathway:
  Drug metabolism - other enzymes (hsa00983)
  Metabolic pathways (hsa01100)
  Pyrimidine metabolism (hsa00240)
• Reactome Pathway: Pyrimidine salvage (R-HSA-73614)

Molecular Interaction Atlas (MIA) of This DME

1 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Uridine	DMQTREB	Depression	6A70-6A7Z	Approved	[1]

1 Investigative Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
cytidine	DMK0W8E	Discovery agent	N.A.	Investigative	[1]

Experimental Enzyme Kinetic Data of Drugs

Drug Name	Indication	Highest Status	Kinetic Data	REF
cytidine	Discovery agent [N.A.]	Investigative	Km = 0.086 microM	[1]
Uridine	Depression [6A70-6A7Z]	Approved	Km = 0.05 microM	[1]

References

• [1] Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases. Mol Pharmacol. 2001 May;59(5):1181-6.