Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEZNP5G)

• DME Name: Cytochrome P450 51B1 (cyp51)
• Synonyms: Cytochrome P450 family 51 subfamily B member 1; Cytochrome cyp51B1; MMAR_4932; P450 51B1; cyp51B1
• Gene Name: cyp51B1
• UniProt ID: B2HH81_MYCMM
• INTEDE ID: DME1235
• EC Number:
  EC: 1.14.14.154
  Oxidoreductase
  Oxygen paired donor oxidoreductase
  Flavin/flavoprotein donor oxidoreductase
  EC: 1.14.14.154
• Lineage:
  Species: Mycobacterium tuberculosis
  Kingdom: Bacteria
  Phylum: Actinobacteria
  Class: Actinobacteria
  Order: Corynebacteriales
  Family: Mycobacteriaceae
  Genus: Mycobacterium
  Species: Mycobacterium tuberculosis
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MTTAIVPRVSGGEEEHGHLEEFRTDPIGLMQRVRDECGDVGWFQLANKHVVLLSGAKANE
  FFFRSSDEELDQAEAYPFMTPIFGKGVVFDASPERRKEMLHNSALRGEHMKGHATTIERE
  VHRMIENWGQEGEIDLLEFFAELTIYTSTSCLIGTKFRNQLDSRFAHFYHELERGTDPLC
  YVDPYLPIESFRRRDEARKGLVALVQDIMHQRVANPPTDKRDRDMLDVLVSITDEQGNPR
  FCADEVTGMFISLMFAGHHTSSGTSAWTLIELLRHPDAYAAVIDELDELYADGQPVSFHA
  LRQIPRLENVLKETLRLHPPLIILMRVAKGEFQVEGYPIHEGELVAASPAISNRIAEDFP
  DPDEFVPERYQEPRQEDLINRWTWIPFGAGRHRCVGAAFATMQIKAIFSVLLREYEFEMA
  QPADSYRNDHSKMVVQLARPARVRYRRRKMSDNRGH
• Function: This enzyme is cytochrome P-450 (heme-thiolate) protein acting on a range of steroids with a 14alpha-methyl group, such as obtusifoliol and lanosterol. And it catalyses a hydroxylation and a reduction of the 14alpha-methyl group, followed by a second hydroxylation, resulting in the elimination of formate and formation of a 14(15) double bond.
• KEGG Pathway:
  Biosynthesis of secondary metabolites (mmi01110)
  Metabolic pathways (mmi01100)
  Steroid biosynthesis (mmi00100)

Molecular Interaction Atlas (MIA) of This DME

2 Investigative Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Dihydrolanosterol	DMG0PXI	N. A.	N. A.	Investigative	[1]
Lanosterol	DMHN74V	Discovery agent	N.A.	Investigative	[1]

References

• [1] Function, essentiality, and expression of cytochrome P450 enzymes and their cognate redox partners in Mycobacterium tuberculosis: are they drug targets? Appl Microbiol Biotechnol. 2019 May;103(9):3597-3614.