Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE4AMLP)

• DME Name: Nicotinate dehydrogenase (nicA)
• Synonyms: Nicotinate degradation protein A; Nicotinate dehydrogenase small subunit; PP_3947; ndhS; nicA
• Gene Name: nicA
• UniProt ID: NICA_PSEPK
• INTEDE ID: DME1069
• Gene ID: 1046617
• EC Number:
  EC: 1.17.2.1
  Oxidoreductase
  CH/CH2 oxidoreductase
  MCD acceptor oxidoreductase
  EC: 1.17.2.1
• Lineage:
  Species: Pseudomonas putida
  Kingdom: Bacteria
  Phylum: Proteobacteria
  Class: Gammaproteobacteria
  Order: Pseudomonadales
  Family: Pseudomonadaceae
  Genus: Pseudomonas
  Species: Pseudomonas putida
  Subspecies: Pseudomonas putida S16
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MQTTISLQVNGQPVEVSAMPDTPLLLILRNDLCLNGPKYGCGLGECGACTVIIDGVAARS
  CVIPLAGAAGRNITTLEGLGSKAAPHPVQQAFIDEQAAQCGYCMNGMIMTAKALLDRIPE
  PSDEQIRNELSANLCRCGTHVEILRAVRRAAETRRKP
• Function: This enzyme is subunit of the two-component enzyme NicAB that mediates nicotinate hydroxylation, the first step in the aerobic nicotinate degradation pathway. And it mediates conversion of nicotinate into 6-hydroxynicotinate (6HNA).
• KEGG Pathway:
  Metabolic pathways (ppu01100)
  Microbial metabolism in diverse environments (ppu01120)
  Nicotinate and nicotinamide metabolism (ppu00760)

Molecular Interaction Atlas (MIA) of This DME

1 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Nicotine	DMWX5CO	Lung cancer	2C25.0	Approved	[1]

Experimental Enzyme Kinetic Data of Drugs

Drug Name	Indication	Highest Status	Kinetic Data	REF
Nicotine	Lung cancer [2C25.0]	Approved	Km = 0.24 microM	[1]

References

• [1] Optimization of a nicotine degrading enzyme for potential use in treatment of nicotine addiction. BMC Biotechnol. 2019 Aug 2;19(1):56.