Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE6PKFB)

• DME Name: Rifampicin monooxygenase (rox)
• Synonyms: Monooxygenase rifampicin; RIF-O; RIFMO; RifMO; rox; roxNFA_35380
• Gene Name: rox
• UniProt ID: ROX_NOCFA
• INTEDE ID: DME1294
• EC Number:
  EC: 1.14.13.-
  Oxidoreductase
  Oxygen paired donor oxidoreductase
  NADH/NADPH donor oxidoreductase
  EC: 1.14.13.-
• Lineage:
  Species: Nocardia farcinica
  Kingdom: Bacteria
  Phylum: Actinobacteria
  Class: Actinobacteria
  Order: Corynebacteriales
  Family: Nocardiaceae
  Genus: Nocardia
  Species: Nocardia farcinica
• Tissue Distribution: Primarily distributed in human lung.
• Sequence:
  MIDVIIAGGGPTGLMLAGELRLHGVRTVVLEKEPTPNQHSRSRGLHARSIEVMDQRGLLE
  RFLAHGEQFRVGGFFAGLAAEWPADLDTAHSYVLAIPQVVTERLLTEHATELGAEIRRGC
  EVAGLDQDADGVTAELADGTRLRARYLVGCDGGRSTVRRLLGVDFPGEPTRVETLLADVR
  IDVPVETLTAVVAEVRKTQLRFGAVPAGDGFFRLIVPAQGLSADRAAPTLDELKRCLHAT
  AGTDFGVHSPRWLSRFGDATRLAERYRTGRVLLAGDAAHIHPPTGGQGLNLGIQDAFNLG
  WKLAAAIGGWAPPDLLDSYHDERHPVAAEVLDNTRAQMTLLSLDPGPRAVRRLMAELVEF
  PDVNRHLIEKITAIAVRYDLGDGHDLVGRRLRDIPLTEGRLYERMRGGRGLLLDRTGRLS
  VSGWSDRVDHLADPGAALDVPAALLRPDGHVAWVGEDQDDLLAHLPRWFGAAT
• Function: This enzyme can modify rifampicin, thereby inactivating its antibiotic activity. And it constitutes a secondary rifampicin resistance factor.

Molecular Interaction Atlas (MIA) of This DME

1 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Rifampicin	DM5DSFZ	Non-insulin dependent diabetes	5A11	Approved	[1]

References

• [1] Mechanism of rifampicin inactivation in Nocardia farcinica. PLoS One. 2016 Oct 5;11(10):e0162578.