Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE87ZNW)

• DME Name: Cytochrome P450 121A1 (cyp121)
• Synonyms: Cytochrome P450 family 121 subfamily A member 1; Cytochrome P450 121; Cytochrome P450 MT2; MTCY339.34c; Mycocyclosin synthase; Rv2276; cyp121
• Gene Name: cyp121
• UniProt ID: CP121_MYCTU
• INTEDE ID: DME1662
• Gene ID: 888373
• EC Number:
  EC: 1.14.19.70
  Oxidoreductase
  Oxygen paired donor oxidoreductase
  Oxygen paired donor oxidoreductase
  EC: 1.14.19.70
• Lineage:
  Species: Mycobacterium tuberculosis
  Kingdom: Bacteria
  Phylum: Actinobacteria
  Class: Actinobacteria
  Order: Corynebacteriales
  Family: Mycobacteriaceae
  Genus: Mycobacterium
  Species: Mycobacterium tuberculosis
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MTATVLLEVPFSARGDRIPDAVAELRTREPIRKVRTITGAEAWLVSSYALCTQVLEDRRF
  SMKETAAAGAPRLNALTVPPEVVNNMGNIADAGLRKAVMKAITPKAPGLEQFLRDTANSL
  LDNLITEGAPADLRNDFADPLATALHCKVLGIPQEDGPKLFRSLSIAFMSSADPIPAAKI
  NWDRDIEYMAGILENPNITTGLMGELSRLRKDPAYSHVSDELFATIGVTFFGAGVISTGS
  FLTTALISLIQRPQLRNLLHEKPELIPAGVEELLRINLSFADGLPRLATADIQVGDVLVR
  KGELVLVLLEGANFDPEHFPNPGSIELDRPNPTSHLAFGRGQHFCPGSALGRRHAQIGIE
  ALLKKMPGVDLAVPIDQLVWRTRFQRRIPERLPVLW
• Function: This enzyme is a P-450 heme-thiolate protein, and it catalyzes C-C bond formation between the carbons ortho to the phenolic hydroxyl of cyclo(L-tyr-L-tyr) (cYY) producing mycocyclosin. The enzme also can use cyclo(L-Tyr-L-Phe) (cYF), cyclo(L-Tyr-L-Trp) (cYW) and cyclo(L-Tyr-L-3,4-dihydroxyphenylalanine) (cY-DOPA) as substrate.

Molecular Interaction Atlas (MIA) of This DME

1 Investigative Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
phytanic acid	DMSZ1RB	Discovery agent	N.A.	Investigative	[1]

References

• [1] Function, essentiality, and expression of cytochrome P450 enzymes and their cognate redox partners in Mycobacterium tuberculosis: are they drug targets? Appl Microbiol Biotechnol. 2019 May;103(9):3597-3614.