Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEC389G)

• DME Name: Alpha-L-rhamnosidase (lrA)
• Synonyms: Alpha-L-rhamnosidase T; Alpha-L-rhamnosidase N; Alpha-L-rhamnoside rhamnohydrolase; DOE78_02545
• Gene Name: DOE78_02545
• UniProt ID: A0A385NJR4_9BACI
• INTEDE ID: DME1480
• EC Number:
  EC: 3.2.1.40
  Hydrolases
  Glycosylase
  O/S-glycosyl compound glycosidase
  EC: 3.2.1.40
• Lineage:
  Species: Bacillus subtilis
  Kingdom: Bacteria
  Phylum: Firmicutes
  Class: Bacilli
  Order: Bacillales
  Family: Bacillaceae
  Genus: Bacillus
  Species: Bacillus subtilis
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MSFKRSLIIVLSFLLTFSLLYPVTETVEASPPGTITNLQVEYMENPIGVDSENPRFSWNM
  VSNVRGAKQLAYELVIADSEKNLKQGEYVWSSRKVTSSQSVNVKYTGEAKLTASTRYFWK
  VKVWDQDNKVLESKQNAFFETGLMSKDGTTNWDGAQWITNNKAATDPVNVTLFRTEQKVT
  NKPIQNAQLYISSLGIYEAFINGKEVQLLQGQEQAVDETFNPGWTDYNSYVNYQAYDVTS
  YLSNKSHLSLGVMVGKGWYAGRIGQVGQYTKVSGNKLALLAKLVITYKDGSTDVIVTNES
  NWKSSEKSPIKSNDFFDGETYDAIVAKEIEGWNNVKYDDSEWSPVSSFIYKGEVKGSRAA
  AARVAEEYTQQPIGAYKYSEIEQSVQNGGESSYDFGHAITTKVKPNSTIHLKAGEILIVD
  MGQNMAGVPNIKVSGDTGSTVTMRFAEMINDGKKNPKIMSGGSDGPKDTLYRKALTSAAQ
  TDKYILSGQGVEEYQPVFTFHGYRYMEVTADADLRIHSAEGKVITSVKDQTGDITTSNSD
  VNKLFSNVLWGQRSNYLSIPTDSPQRAERAGWTGDAQLFAQSAVYNYNDFSFLENYSEIM
  DTHNKNNNGIYGSIMPGAFVGFFANTVASGWSDSGIIIPWVLYQQTGDTTVIEKNFAQME
  KYMDYVEKSGYNTGLFGDWLAFQATSTVYLNTIYRAYDAQLMAKMAKVIGNETAVDKYEE
  LYAKTKSDFVNKYFDDKGNLLTASADNFTQSRHGYPGADNAQTGLLWALKLGLYETEQQR
  ETMVQNLTANIKNEGSLIRPGQPENTLAVGFLGVNVLLPTLSDIGDNDLAYTLLLQDEMP
  SWLYSVKNGATTIWERWNSYSIEDSFGDSGMNSFNHYSYGAVAEWMYKYMAGISNDPEKP
  GFQHFVLQPTIDGENRISWVNGSYDSVYGEIKSNWKVENGTFKYQVTIPANTSATLAIPA
  TNVNEVTEGGVSLSKAKGIKVIEVKDGKVYCEVSSGTYDITSKVN
• Function: This enzyme utilizes an inverting mechanism of hydrolysis, releasing beta-L-rhamnose. Substrates include naringin, rutin, quercitrin, hesperidin, dioscin, terpenyl glycosides and many other natural glycosides containing terminal alpha-L-rhamnose.

Molecular Interaction Atlas (MIA) of This DME

1 Investigative Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Rutin	DMEHRAJ	Discovery agent	N.A.	Investigative	[1]

References

• [1] Identification of rutin deglycosylated metabolites produced by human intestinal bacteria using UPLC-Q-TOF/MS. J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jun 1;898:95-100.