Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEFYE6A)

• DME Name: L-fucose isomerase (fucI)
• Synonyms: Isomerase L-fucose; D-arabinose isomerase; FucIase; 6-deoxy-L-galactose isomerase; fucI
• Gene Name: fucI
• UniProt ID: A0A5C0EBW9_9ENTR
• INTEDE ID: DME1636
• EC Number:
  EC: 5.3.1.25
  Isomerase
  Intramolecular oxidoreductase
  Aldose/ketose isomerase
  EC: 5.3.1.25
• Lineage:
  Species: Raoultella planticola
  Kingdom: Bacteria
  Phylum: Proteobacteria
  Class: Gammaproteobacteria
  Order: Enterobacterales
  Family: Enterobacteriaceae
  Genus: Raoultella
  Species: Raoultella planticola
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MKRISLPKIGIRPVIDGRRMGVRESLEAQTMNMAKATAALISEKLRHACGAQIECVIADT
  CIAGMAESAACEEKFSRQNVGVTITVTPCWCYGSETIDMDPLRPKAIWGFNGTERPGAVY
  LAAALAAHSQKGIPAFSIYGHDVQDADDTTIPADVEEKLLRFARAGLAVASMKGKSYLSV
  GGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRIDQKIYDEVELEMALAWADKNFRY
  GEDQNAQHYKRDEEQSRAVLKESLLMAMCIRDMMQGNEKLAEKGLLEESLGYNAIAAGFQ
  GQRHWTDQYPNGDTAEALLNSSFDWNGVREPFVVATENDSLNGVAMLMGHQLTGTAQVFA
  DVRTYWSPDAVERVTGQPLTGLAEHGIIHLINSGSAALDGSCQQRDEEGKPTMKPHWEIS
  QKEADACLAATEWCPAIHEYFRGGGYSSRFLTEGGVPFTMTRVNLIKGLGPVLQIAEGWS
  VELPKAMHDQLDARTNSTWPTTWFAPRLTGKGPFADVYSVMANWGANHGVLTIGHVGADF
  ITLAAMLRIPVCMHNVEEGKIYRPSSWSAHGMDTEGQDYRACQNYGPLYKR
• Function: This enzyme converts the aldose L-fucose into the corresponding ketose L-fuculose. It requires a divalent metal ion and binds the closed form of the sugar and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism.

Molecular Interaction Atlas (MIA) of This DME

1 Clinical Trial Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
CERC-803	DMUW6KP	N. A.	N. A.	Phase 1/2	[1], [2]

References

• [1] Enzymatic synthesis of l-fucose from l-fuculose using a fucose isomerase from Raoultella sp. and the biochemical and structural analyses of the enzyme. Biotechnol Biofuels. 2019 Dec 5;12:282.
• [2] Raoultella spp.-clinical significance, infections and susceptibility to antibiotics. Folia Microbiol (Praha). 2017 May;62(3):221-227.