Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEU37LK)

• DME Name: L-arabinose isomerase (araA)
• Synonyms: Isomerase L-arabinose; D-arabinose aldose-ketose-isomerase; araA; CLOHYLEM_05792; LAJLEIBI_03201
• Gene Name: araA
• UniProt ID: C0C0X3_9FIRM
• INTEDE ID: DME1054
• Gene ID: 42452782
• EC Number:
  EC: 5.3.1.4
  Isomerase
  Intramolecular oxidoreductase
  Aldose/ketose isomerase
  EC: 5.3.1.4
• Lineage:
  Species: Clostridium hylemonae
  Kingdom: Bacteria
  Phylum: Firmicutes
  Class: Clostridia
  Order: Clostridiales
  Family: Lachnospiraceae
  Genus: Lachnoclostridium
  Species: Clostridium hylemonae
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MIKSKEYKFWFCTGSQDLYGEECLAHVAEHSRRIVEALNASGALPYEVVWKPTLITNELI
  RKTFNEANTDETCAGVITWMHTFSPAKSWILGLQEYRKPLLHLHTQFNEEIPYDTIDMDF
  MNENQAAHGDREYGHIFSRLRMERKVVAGYWADPKVQKKIGSWMRTAVGVIESSHVRVMR
  IADNMRNVAVTEGDKVEAQIKFGWEVDTYPVNEIAEAVAEVSKSDIGTLVEEYYDKYDIL
  LEGRDEKEFREHVAVQAGIEIGFERFLEERDYQAIVTHFGDLGSLKQLPGLAIQRLMEKG
  YGFGGEGDWKTAAMVRIMKIMTEGVKDARGTSFMEDYTYNLIPGKEGILQAHMLEVCPSV
  AEGPISIKCQPLTMGDREDPARLVFTSKEGPAVAASLIDLGDRFRLIINDVDCKKTEKPM
  PKLPVATAFWTPQPDLQTGAEAWILAGGAHHTAFSYDLSAGQLGDWADAMGIEAVYIDKN
  TEIRQFKNELRWNAAAYKLGI
• Function: This enzyme catalyzes the conversion of L-arabinose to L-ribulose. It requires a divalent metal ion and binds the closed form of the sugar and catalyses ring opening to generate a form of open-chain conformation that facilitates the isomerization reaction, which proceeds via an ene-diol mechanism. It can also convert D-galactose to D-tagatose with lower efficiency.

Molecular Interaction Atlas (MIA) of This DME

1 Clinical Trial Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
CERC-801	DM3SZ7P	N. A.	N. A.	Phase 2	[1]

References

• [1] Biochemical properties of L-arabinose isomerase from Clostridium hylemonae to produce D-tagatose as a functional sweetener. PLoS One. 2018 Apr 23;13(4):e0196099.