Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEU1RXB)

• DME Name: Beta-lactamase (blaB)
• Synonyms: Penicillinase; Cephalosporinase; Beta-lactam; cfxA
• Gene Name: cfxA
• UniProt ID: BLAC_BACVU
• INTEDE ID: DME1220
• EC Number:
  EC: 3.5.2.6
  Hydrolases
  Carbon-nitrogen hydrolase
  Cyclic amide hydrolase
  EC: 3.5.2.6
• Lineage:
  Species: Bacteroides vulgatus
  Kingdom: Bacteria
  Phylum: Bacteroidetes
  Class: Bacteroidia
  Order: Bacteroidales
  Family: Bacteroidaceae
  Genus: Bacteroides
  Species: Bacteroides vulgatus
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MEKNRKKQIVVLSIALVCIFILVFSLFHKSATKDSANPPLTNVLTDSISQIVSACPGEIG
  VAVIVNNRDTVKVNNKSVYPMMSVFKVHQALALCNDFDNKGISLDTLVNINRDKLDPKTW
  SPMLKDYSGPVISLTVRDLLRYTLTQSDNNASNLMFKDMVNVAQTDSFIATLIPRSSFQI
  AYTEEEMSADHNKAYSNYTSPLGAAMLMNRLFTEGLIDDEKQSFIKNTLKECKTGVDRIA
  APLLDKEGVVIAHKTGSGYVNENGVLAAHNDVAYICLPNNISYTLAVFVKDFKGNKSQAS
  QYVAHISAVVYSLLMQTSVKS
• Function: This enzyme hydrolyze cephalosporins, penicillins and also cefoxitin; but at a slow rate.

Molecular Interaction Atlas (MIA) of This DME

3 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Amoxicillin	DMUYNEI	Acute otitis media	AB00	Approved	[1], [2]
Cefotaxime	DMEB837	Bacteremia	1A73	Approved	[3]
Cefoxitin	DMY8NC4	Acute gonococcal cervicitis		Approved	[3]

References

• [1] Prevalence of antimicrobial resistance genes in Bacteroides spp. and Prevotella spp. Dutch clinical isolates. Clin Microbiol Infect. 2019 Sep;25(9):1156.e9-1156.e13.
• [2] Beta-lactamase production, beta-lactam sensitivity and resistance to synergy with clavulanate of 737 Bacteroides fragilis group organisms from thirty-three US centres. J Antimicrob Chemother. 1990 Sep;26(3):361-70.
• [3] Presence of the cfxA gene in Bacteroides distasonis. Res Microbiol. 2003 Jun;154(5):369-74.