Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE3FVRW)

• DME Name: Beta-lactamase (blaB)
• Synonyms: Penicillinase; Cephalosporinase; Beta-lactam; Metallo-beta-lactamase type 2a; Metallo-beta-lactamase type IIa; Metallothioprotein beta-lactamase IIa; Zinc-requiring beta-lactamase Iia
• Gene Name: blaB
• UniProt ID: BLA2_BAC17
• INTEDE ID: DME1214
• EC Number:
  EC: 3.5.2.6
  Hydrolases
  Carbon-nitrogen hydrolase
  Cyclic amide hydrolase
  EC: 3.5.2.6
• Lineage:
  Species: Bacillus megaterium
  Kingdom: Bacteria
  Phylum: Firmicutes
  Class: Bacilli
  Order: Bacillales
  Family: Bacillaceae
  Genus: Bacillus
  Species: Bacillus megaterium
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MKKNTLLKVGLCVSLLGTTQFVSTISSVQASQKVEQIVIKNETGTISISQLNKNVWVHTE
  LGYFNGEAVPSNGLVLNTSKGLVLVDSSWDNKLTKELIEMVEKKFQKRVTDVIITHAHAD
  RIGGITALKERGIKAHSTALTAELAKKSGYEEPLGDLQTVTNLKFGNTKVETFYPGKGHT
  EDNIVVWLPQYQILAGGCLVKSAEAKNLGNVADAYVNEWSTSIENMLKRYRNINLVVPGH
  GKVGDKGLLLHTLDLLK
• Function: This enzyme confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring.

Molecular Interaction Atlas (MIA) of This DME

2 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Ampicillin	DMHWE7P	Acute epiglottitis		Approved	[1]
Ceftiofur	DMR2SOZ	Bacterial infection	1A00-1C4Z	Approved	[2], [3]

References

• [1] Beta-lactamase production and antimicrobial susceptibility of subgingival bacteria from refractory periodontitis. Oral Microbiol Immunol. 2004 Oct;19(5):303-8.
• [2] The bacterial degradation of chloramphenico. Lancet. 1967 Jun 10;1(7502):1259-60.
• [3] Isolation of bacterial strains from bovine fecal microflora capable of degradation of ceftiofur. Vet Microbiol. 2009 Oct 20;139(1-2):89-96.