Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE21PLI)

• DME Name: NADPH-dependent nitroreductase (nfrA1)
• Synonyms: NADPH-dependent FMN reductase; NADPH-dependent nitro/flavin reductase; NADPH-dependent oxidoreductase; FMN reductase (NADPH); BSU38110; ipa-43d; nfrA1; ywcG
• Gene Name: nfrA1
• UniProt ID: NFRA1_BACSU
• INTEDE ID: DME1243
• Gene ID: 937279
• EC Number:
  EC: 1.5.1.38
  Oxidoreductase
  CH-NH donor oxidoreductase
  NAD/NADP acceptor oxidoreductase
  EC: 1.5.1.38
• Lineage:
  Species: Bacillus subtilis
  Kingdom: Bacteria
  Phylum: Firmicutes
  Class: Bacilli
  Order: Bacillales
  Family: Bacillaceae
  Genus: Bacillus
  Species: Bacillus subtilis
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MNNTIETILNHRSIRSFTDQLLTAEEIDTLVKSAQAASTSSYVQAYSIIGVSDPEKKREL
  SVLAGNQPYVEKNGHFFVFCADLYRHQQLAEEKGEHISELLENTEMFMVSLIDAALAAQN
  MSIAAESMGLGICYIGGIRNELDKVTEVLQTPDHVLPLFGLAVGHPANLSGKKPRLPKQA
  VYHENTYNVNTDDFRHTMNTYDKTISDYYRERTNGKREETWSDQILNFMKQKPRTYLNDY
  VKEKGFNKN
• Function: This enzyme can reduce FMNH(2) to FMN, with NADPH as reductant, and also can reduce nitroaromatic compounds, quinones and azo dyes.
• KEGG Pathway:
  Metabolic pathways (bsu01100)
  Riboflavin metabolism (bsu00740)

Molecular Interaction Atlas (MIA) of This DME

3 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Nitrofurantoin	DM7PQIK	Urinary tract infection	GC08	Approved	[1], [2]
Nitrofurazone	DMSM2KE	Skin burns	ME65.0	Approved	[1], [2]
Riboflavin	DM8YMWE	Acne vulgaris	ED80	Approved	[1]

References

• [1] Conversion of NfsA, the major Escherichia coli nitroreductase, to a flavin reductase with an activity similar to that of Frp, a flavin reductase in Vibrio harveyi, by a single amino acid substitution. J Bacteriol. 1998 Jan;180(2):422-5.
• [2] Bacillus subtilis isolated from the human gastrointestinal tract. Res Microbiol. 2009 Mar;160(2):134-43.