Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEMIUB2)

• DME Name: Beta-lactamase (blaB)
• Synonyms: Penicillinase; Cephalosporinase; Beta-lactam; Beta-lactamase TEM; IRT-4; TEM-1; TEM-16/CAZ-7; TEM-2; TEM-24/CAZ-6; TEM-3; TEM-4; TEM-5; TEM-6; TEM-8/CAZ-2; and; bla; blaT-3; blaT-4; blaT-5; blaT-6
• Gene Name: bla
• UniProt ID: BLAT_ECOLX
• INTEDE ID: DME1223
• Gene ID: 10076131
• EC Number:
  EC: 3.5.2.6
  Hydrolases
  Carbon-nitrogen hydrolase
  Cyclic amide hydrolase
  EC: 3.5.2.6
• Lineage:
  Species: Escherichia coli
  Kingdom: Bacteria
  Phylum: Proteobacteria
  Class: Gammaproteobacteria
  Order: Enterobacterales
  Family: Enterobacteriaceae
  Genus: Escherichia
  Species: Escherichia coli
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRP
  EERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVREL
  CSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTM
  PAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGS
  RGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW
• Function: This enzyme is the most prevalent beta-lactamase in enterobacteria. It hydrolyzes the beta-lactam bond in susceptible beta-lactam antibiotics, thus conferring resistance to penicillins and cephalosporins. TEM-3 and TEM-4 are capable of hydrolyzing cefotaxime and ceftazidime. TEM-5 is capable of hydrolyzing ceftazidime. TEM-6 is capable of hydrolyzing ceftazidime and aztreonam. TEM-8/CAZ-2, TEM-16/CAZ-7 and TEM-24/CAZ-6 are markedly active against ceftazidime. IRT-4 shows resistance to beta-lactamase inhibitors.

Molecular Interaction Atlas (MIA) of This DME

4 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Cefotaxime	DMEB837	Bacteremia	1A73	Approved	[1]
Cefoxitin	DMY8NC4	Acute gonococcal cervicitis		Approved	[1]
Ertapenem	DM4S3O8	Bacterial infection	1A00-1C4Z	Approved	[2]
Cefpirome	DMJKWPZ	N. A.	N. A.	Phase 4	[1]

References

• [1] Oral streptococcal strains isolated from odontogenic infections and their susceptibility to antibiotics. Rev Med Chir Soc Med Nat Iasi. 2006 Oct-Dec;110(4):1012-5.
• [2] War wound treatment complications due to transfer of an IncN plasmid harboring bla(OXA-181) from Morganella morganii to CTX-M-27-producing sequence type 131 Escherichia coli. Antimicrob Agents Chemother. 2015;59(6):3556-62.