Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DET9I1W)

• DME Name: Beta-lactamase (blaB)
• Synonyms: Penicillinase; Cephalosporinase; Beta-lactam; cblA
• Gene Name: cblA
• UniProt ID: BLAC_BACUN
• INTEDE ID: DME1026
• Gene ID: 31505337
• EC Number:
  EC: 3.5.2.6
  Hydrolases
  Carbon-nitrogen hydrolase
  Cyclic amide hydrolase
  EC: 3.5.2.6
• Lineage:
  Species: Bacteroides uniformis
  Kingdom: Bacteria
  Phylum: Bacteroidetes
  Class: Bacteroidia
  Order: Bacteroidales
  Family: Bacteroidaceae
  Genus: Bacteroides
  Species: Bacteroides uniformis
  Subspecies: Bacteroides uniformis B 371
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MKAYFIAILTLFTCIATVVRAQQMSELENRIDSLLNGKKATVGIAVWTDKGDMLRYNDHV
  HFPLLSVFKFHVALAVLDKMDKQSISLDSIVSIKASQMPPNTYSPLRKKFPDQDFTITLR
  ELMQYSISQSDNNACDILIEYAGGIKHINDYIHRLSIDSFNLSETEDGMHSSFEAVYRNW
  STPSAMVRLLRTADEKELFSNKELKDFLWQTMIDTETGANKLKGMLPAKTVVGHKTGSSD
  RNADGMKTADNDAGLVILPDGRKYYIAAFVMDSYETDEDNANIIARISRMVYDAMR
• Function: This enzyme hydrolyzes beta-lactam.

Molecular Interaction Atlas (MIA) of This DME

4 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Amoxicillin	DMUYNEI	Acute otitis media	AB00	Approved	[1]
Ampicillin	DMHWE7P	Acute epiglottitis		Approved	[2]
Cefalotin	DMVYSLE	Bacteremia	1A73	Approved	[2]
Piperacillin	DMTBKF3	Acute gonococcal cervicitis		Approved	[2]

1 Clinical Trial Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Benzylpenicillin	DMS9503	Actinomycosis		Phase 3	[2]

1 Discontinued Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Cephaloridine	DM4Y95F	Gram-positive bacterial infection	1B74-1G40	Withdrawn from market	[2]

Experimental Enzyme Kinetic Data of Drugs

Drug Name	Indication	Highest Status	Kinetic Data	REF
Cephaloridine	Gram-positive bacterial infection [1B74-1G40]	Withdrawn from market	Km = 177 microM	[2]
Benzylpenicillin	Actinomycosis []	Phase 3	Km = 3.5 microM	[2]
Ampicillin	Acute epiglottitis []	Approved	Km = 4.4 microM	[2]
Cefalotin	Bacteremia [1A73]	Approved	Km = 113 microM	[2]
Piperacillin	Acute gonococcal cervicitis []	Approved	Km = 0.58 microM	[2]

References

• [1] Prevalence of antimicrobial resistance genes in Bacteroides spp. and Prevotella spp. Dutch clinical isolates. Clin Microbiol Infect. 2019 Sep;25(9):1156.e9-1156.e13.
• [2] Purification and characterization of a new beta-lactamase from Bacteroides uniformis. Antimicrob Agents Chemother. 1995 Jul;39(7):1458-61.