Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE0QLUZ)

DME Name	Oxygen-insensitive NADPH nitroreductase B (nfsB)
Synonyms	Oxygen-insensitive NAD(P)H nitroreductase B; Dihydropteridine reductase; FMN-dependent nitroreductase; JW0567; b0578; ntr; pnrB
Gene Name	nfsB
UniProt ID	NFSB_ECOLI
INTEDE ID	DME1048
3D Structure	Download 2D Sequence (FASTA) Download 3D Structure (PDB) Download
Gene ID	945778
EC Number	EC: 1.5.1.34 Oxidoreductase CH-NH donor oxidoreductase NAD/NADP acceptor oxidoreductase EC: 1.5.1.34
Lineage	Species: Escherichia coli Kingdom: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Escherichia Species: Escherichia coli
Tissue Distribution	Primarily distributed in human gut.
Sequence	MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARV AKSAAGNYVFNERKMLDASHVVVFCAKTAMDDVWLKLVVDQEDADGRFATPEAKAANDKG RKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLDAVPIEGFDAAILDAEFGLKE KGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV
Function	This enzyme can reduce a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. And it can reduce nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide).
KEGG Pathway	Microbial metabolism in diverse environments (ecj01120 ) Nitrotoluene degradation (ecj00633 )

Molecular Interaction Atlas (MIA) of This DME

Molecular Interaction Atlas (MIA)

Jump to Detail Molecular Interaction Atlas of This DME

4 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Clonazepam	DMTO13J	Absence epilepsy		Approved	[1]
Flunitrazepam	DMGR5Z3	Insomnia	7A00-7A0Z	Approved	[2]
Nitrazepam	DMEGIQ6	Epilepsy	8A60-8A68	Approved	[3]
Nitrofurazone	DMSM2KE	Skin burns	ME65.0	Approved	[3]
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1 Discontinued Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
CB1954	DMVP4YK	Solid tumour/cancer	2A00-2F9Z	Discontinued in Phase 2	[3], [4]
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2 Investigative Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Misonidazole	DMYB0HK	N. A.	N. A.	Investigative	[5]
Nitrobenzodiazepine	DM254EW	N. A.	N. A.	Investigative	[2]
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References

1	Characterization of Escherichia coli nitroreductase NfsB in the metabolism of nitrobenzodiazepines. Biochem Pharmacol. 2009 Jul 1;78(1):96-103.
2	Structure-based development of bacterial nitroreductase against nitrobenzodiazepine-induced hypnosis. Biochem Pharmacol. 2012 Jun 15;83(12):1690-9.
3	Conversion of NfsB, a minor Escherichia coli nitroreductase, to a flavin reductase similar in biochemical properties to FRase I, the major flavin reductase in Vibrio fischeri, by a single amino acid substitution. J Bacteriol. 1996 Aug;178(15):4731-3.
4	Discovery and evaluation of Escherichia coli nitroreductases that activate the anti-cancer prodrug CB1954. Biochem Pharmacol. 2010 Mar 1;79(5):678-87.
5	Metronidazole (flagyl) and misonidazole (Ro-07-0582): reduction by facultative anaerobes and cytotoxic action on hypoxic bacteria and mammalian cells in vivo. Br J Cancer Suppl. 1978 Jun;3:132-5.