Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE5WGIM)

• DME Name: Aminoglycoside phosphotransferase (aph-Ib)
• Synonyms: Campylobacter jejuni aminoglycoside phosphotransferase; 2''-aminoglycoside phosphotransferase; Aph(2'')-Ib
• Gene Name: aph(2'')-Ib
• UniProt ID: A0A075C7U3_CAMJU
• INTEDE ID: DME1090
• EC Number:
  EC: 2.7.1.190
  Transferase
  Kinase
  Phosphotransferase
  EC: 2.7.1.190
• Lineage:
  Species: Campylobacter jejuni
  Kingdom: Bacteria
  Phylum: Proteobacteria
  Class: Epsilonproteobacteria
  Order: Campylobacterales
  Family: Campylobacteraceae
  Genus: Campylobacter
  Species: Campylobacter jejuni
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MIDLDVEIYQHLNEQIKINKLCYLSSGDDSDTFLCNEQYVVKVPKRDSVRFAQKREFELY
  RFLENCNLSYQTPAVVYQSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKEL
  HSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENISSNAV
  IFNYIPCLVHNDFSANNLIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGR
  KVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKGVSELLQTQAEMFIF
• Function: This enzyme is the most clinically important aminoglycoside-modifying enzyme in Gram-positive bacteria, responsible for high-level resistance in both Enterococci and Staphylococci. And this bacterial enzyme phosphorylates many 4,6-disubstituted aminoglycoside antibiotics that have a hydroxyl group at position 2'', including kanamycin A, kanamycin B, tobramycin, dibekacin, arbekacin, amikacin, gentamicin C, sisomicin and netilmicin.

Molecular Interaction Atlas (MIA) of This DME

5 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Gentamicin	DMKINJO	Bacterial infection	1A00-1C4Z	Approved	[1]
Kanamycin	DMITFB8	Pulmonary tuberculosis	1B10.Z	Approved	[1]
Sisomicin	DM92P0F	Cystitis	GC00	Approved	[2]
Tobramycin	DMUI0CH	Bacterial infection	1A00-1C4Z	Approved	[1]
Dibekacin	DMTX0PZ	Respiratory infection	CA07-CA4Z	Phase 4	[2]

References

• [1] Cloning and expression of novel aminoglycoside phosphotransferase genes from Campylobacter and their role in the resistance to six aminoglycosides. Antimicrob Agents Chemother. 2017 Dec 21;62(1). pii: e01682-17.
• [2] Novel aminoglycoside 2''-phosphotransferase identified in a gram-negative pathogen. Antimicrob Agents Chemother. 2013 Jan;57(1):452-7.