Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DE7IH52)

• DME Name: Beta-lactamase (blaB)
• Synonyms: Penicillinase; Cephalosporinase; Beta-lactam; blaC
• Gene Name: ampC
• UniProt ID: AMPC_CITFR
• INTEDE ID: DME1094
• EC Number:
  EC: 3.5.2.6
  Hydrolases
  Carbon-nitrogen hydrolase
  Cyclic amide hydrolase
  EC: 3.5.2.6
• Lineage:
  Species: Citrobacter freundii
  Kingdom: Bacteria
  Phylum: Proteobacteria
  Class: Gammaproteobacteria
  Order: Enterobacterales
  Family: Enterobacteriaceae
  Genus: Citrobacter
  Species: Citrobacter freundii
  Subspecies: Citrobacter freundii ATCC 6879
• Tissue Distribution: Primarily distributed in human gut.
• Sequence:
  MMKKSICCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQAIPGMAVAIIYEGKPYY
  FTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDRIARGEIKLSDPVTKYWPELTGKQ
  WRGISLLHLATYTAGGLPLQIPGDVTDKAELLRFYQNWQPQWTPGAKRLYANSSIGLFGA
  LAVKSSGMSYEEAMTRRVLQPLKLAHTWITVPQSEQKNYAWGYLEGKPVHVSPGQLDAEA
  YGVKSSVIDMARWVQANMDASHVQEKTLQQGIELAQSRYWRIGDMYQGLGWEMLNWPLKA
  DSIINGSDSKVALAALPAVEVNPPAPAVKASWVHKTGSTGGFGSYVAFVPEKNLGIVMLA
  NKSYPNPARVEAAWRILEKLQ
• Function: This enzyme hydrolyzes beta-lactam with a substrate specificity for cephalosporins.

Molecular Interaction Atlas (MIA) of This DME

6 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Amoxicillin	DMUYNEI	Acute otitis media	AB00	Approved	[1]
Avibactam	DM1AEH4	N. A.	N. A.	Approved	[2], [3]
Cefazolin	DMPDYFR	Bacteremia	1A73	Approved	[4], [3]
Cefotaxime	DMEB837	Bacteremia	1A73	Approved	[4], [2]
Cefoxitin	DMY8NC4	Acute gonococcal cervicitis		Approved	[4]
Imipenem	DMI9FBP	Bacteremia	1A73	Approved	[5]

References

• [1] Detection and characterization of beta-lactamase genes in subgingival bacteria from patients with refractory periodontitis. FEMS Microbiol Lett. 2005 Jan 15;242(2):319-24.
• [2] Analyses of a ceftazidime-avibactam-resistant Citrobacter freundii isolate carrying blaKPC-2 reveals a heterogenous population and reversible genotype. mSphere. 2018 Sep 26;3(5). pii: e00408-18.
• [3] Identification and functional characterization of arylamine N-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid. J Bacteriol. 2001 Jun;183(11):3417-27.
• [4] Citrobacter freundii bacteremia: Risk factors of mortality and prevalence of resistance genes. J Microbiol Immunol Infect. 2018 Aug;51(4):565-572.
• [5] Antibiotic therapy for inducible AmpC beta-lactamase-producing Gram-negative bacilli: what are the alternatives to carbapenems, quinolones and aminoglycosides? Int J Antimicrob Agents. 2012 Oct;40(4):297-305.