Details of Drug-Metabolizing Enzyme (DME)

General Information of Drug-Metabolizing Enzyme (DME) (ID: DEDPI65)

DME Name	Oxygen-insensitive NADPH nitroreductase A (nfsA)
Synonyms	Oxygen-insensitive NAD(P)H nitroreductase A; Modulator of drug activity A; JW0835; b0851; mda18; mdaA; nfsA; pnrA; ybjB
Gene Name	nfsA
UniProt ID	NFSA_ECOLI
INTEDE ID	DME1098
3D Structure	Download 2D Sequence (FASTA) Download 3D Structure (PDB) Download
Gene ID	945483
EC Number	EC: 1.5.1.38 Oxidoreductase CH-NH donor oxidoreductase NAD/NADP acceptor oxidoreductase EC: 1.5.1.38
Lineage	Species: Escherichia coli Kingdom: Bacteria Phylum: Proteobacteria Class: Gammaproteobacteria Order: Enterobacterales Family: Enterobacteriaceae Genus: Escherichia Species: Escherichia coli
Tissue Distribution	Primarily distributed in human gut.
Sequence	MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREEL VTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAE SLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENS YQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR
Function	This enzyme is major oxygen-insensitive nitroreductase in E.coli. And it catalyzes the reduction of nitroaromatic compounds using NADPH, and has a broad electron acceptor specificity. Moreover, it reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product.
KEGG Pathway	Microbial metabolism in diverse environments (ecj01120 ) Nitrotoluene degradation (ecj00633 )

Molecular Interaction Atlas (MIA) of This DME

Molecular Interaction Atlas (MIA)

Jump to Detail Molecular Interaction Atlas of This DME

3 Approved Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
Loperamide	DMOJZQ9	Diarrhea	ME05.1	Approved	[1]
Nitrazepam	DMEGIQ6	Epilepsy	8A60-8A68	Approved	[2]
Nitrofurazone	DMSM2KE	Skin burns	ME65.0	Approved	[2]
------------------------------------------------------------------------------------

1 Clinical Trial Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
HSDB-674	DMJG4WR	N. A.	N. A.	Phase 1	[3]
------------------------------------------------------------------------------------

1 Discontinued Drug(s) Metabolized by This DME

Drug Name	Drug ID	Indication	ICD 11	Highest Status	REF
CB1954	DMVP4YK	Solid tumour/cancer	2A00-2F9Z	Discontinued in Phase 2	[4]
------------------------------------------------------------------------------------

References

1	Reduction of the prodrug loperamide oxide to its active drug loperamide in the gut of rats, dogs, and humans. Drug Metab Dispos. 1995 Mar;23(3):354-62.
2	Biochemical characterization of NfsA, the Escherichia coli major nitroreductase exhibiting a high amino acid sequence homology to Frp, a Vibrio harveyi flavin oxidoreductase. J Bacteriol. 1996 Aug;178(15):4508-14.
3	Absorption of (-)-nicotine-l-N-oxide in man and its reduction in the gastrointestinal tract. J Pharm Pharmacol. 1970 Sep;22(9):722-3.
4	Discovery and evaluation of Escherichia coli nitroreductases that activate the anti-cancer prodrug CB1954. Biochem Pharmacol. 2010 Mar 1;79(5):678-87.