General Information of Drug Therapeutic Target (DTT) (ID: TTIQSC1)

DTT Name Bacterial Lethal factor (Bact lef)
Synonyms lef; Anthrax lethal toxin endopeptidase component; Anthrax lethal factor; ALF
Gene Name Bact lef
DTT Type
Clinical trial target
[1]
BioChemical Class
Peptidase
UniProt ID
LEF_BACAN
TTD ID
T72335
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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EC Number
EC 3.4.24.83
Sequence
MNIKKEFIKVISMSCLVTAITLSGPVFIPLVQGAGGHGDVGMHVKEKEKNKDENKRKDEE
RNKTQEEHLKEIMKHIVKIEVKGEEAVKKEAAEKLLEKVPSDVLEMYKAIGGKIYIVDGD
ITKHISLEALSEDKKKIKDIYGKDALLHEHYVYAKEGYEPVLVIQSSEDYVENTEKALNV
YYEIGKILSRDILSKINQPYQKFLDVLNTIKNASDSDGQDLLFTNQLKEHPTDFSVEFLE
QNSNEVQEVFAKAFAYYIEPQHRDVLQLYAPEAFNYMDKFNEQEINLSLEELKDQRMLAR
YEKWEKIKQHYQHWSDSLSEEGRGLLKKLQIPIEPKKDDIIHSLSQEEKELLKRIQIDSS
DFLSTEEKEFLKKLQIDIRDSLSEEEKELLNRIQVDSSNPLSEKEKEFLKKLKLDIQPYD
INQRLQDTGGLIDSPSINLDVRKQYKRDIQNIDALLHQSIGSTLYNKIYLYENMNINNLT
ATLGADLVDSTDNTKINRGIFNEFKKNFKYSISSNYMIVDINERPALDNERLKWRIQLSP
DTRAGYLENGKLILQRNIGLEIKDVQIIKQSEKEYIRIDAKVVPKSKIDTKIQEAQLNIN
QEWNKALGLPKYTKLITFNVHNRYASNIVESAYLILNEWKNNIQSDLIKKVTNYLVDGNG
RFVFTDITLPNIAEQYTHQDEIYEQVHSKGLYVPESRSILLHGPSKGVELRNDSEGFIHE
FGHAVDDYAGYLLDKNQSDLVTNSKKFIDIFKEEGSNLTSYGRTNEAEFFAEAFRLMHST
DHAERLKVQKNAPKTFQFINDQIKFIINS
Function
One of the three proteins composing the anthrax toxin, the agent which infects many mammalian species and that may cause death. LF is the lethal factor that, when associated with PA, causes death. LF is not toxic by itself. It is a protease that cleaves the N-terminal of most dual specificity mitogen-activated protein kinase kinases (MAPKKs or MAP2Ks) (except for MAP2K5). Cleavage invariably occurs within the N-terminal proline-rich region preceding the kinase domain, thus disrupting a sequence involved in directing specific protein-protein interactions necessary for the assembly of signaling complexes. There may be other cytosolic targets of LF involved in cytotoxicity. The proteasome may mediate a toxic process initiated by LF in the cell cytosol involving degradation of unidentified molecules that are essential for macrophage homeostasis. This is an early step in LeTx intoxication, but it is downstream of the cleavage by LF of MEK1 or other putative substrates.
KEGG Pathway
NOD-like receptor signaling pathway (bar04621 )
Reactome Pathway
Uptake and function of anthrax toxins (R-HSA-5210891 )

Molecular Interaction Atlas (MIA) of This DTT

Molecular Interaction Atlas (MIA) Jump to Detail Molecular Interaction Atlas of This DTT
1 Clinical Trial Drug(s) Targeting This DTT
Drug Name Drug ID Indication ICD 11 Highest Status REF
IQ-DAA DMHP71R Bacillus anthracis infection 1G40 Phase 1 [1]
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1 Discontinued Drug(s) Targeting This DTT
Drug Name Drug ID Indication ICD 11 Highest Status REF
GM6001 DM7V9CT Corneal ulcer 9A76 Discontinued in Phase 2 [2]
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12 Investigative Drug(s) Targeting This DTT
Drug Name Drug ID Indication ICD 11 Highest Status REF
3-(2,5-dimethyl-1H-pyrrol-1-yl)benzoic acid DMUDW1J Discovery agent N.A. Investigative [3]
Bisindolylmaleimide-I DMOQJZC Discovery agent N.A. Investigative [4]
GNF-PF-600 DMWGXBF Discovery agent N.A. Investigative [5]
MMI270 DM38N2K Discovery agent N.A. Investigative [6]
N,N'-Bis(4-Amino-2-Methylquinolin-6-Yl)Urea DMT4USP Discovery agent N.A. Investigative [2]
N-(3,4,5-trihydroxyphenethyl)oleamide DMQ5USA Discovery agent N.A. Investigative [7]
N-(3,4-dihydroxybenzyl)oleamide DM67XSW Discovery agent N.A. Investigative [8]
N-(Sulfanylacetyl)Tyrosylprolylmethioninamide DMWJDYZ Discovery agent N.A. Investigative [2]
N-hydroxy-4-(2-oleamidoethyl)benzamide DMKELRB Discovery agent N.A. Investigative [7]
N-hydroxy-4-(oleamidomethyl)benzamide DMUSHZK Discovery agent N.A. Investigative [8]
N-oleoyl-dopamine DM1AVCZ Discovery agent N.A. Investigative [7]
NSC-622445 DME35CB Discovery agent N.A. Investigative [3]
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⏷ Show the Full List of 12 Investigative Drug(s)

References

1 Anti-toxin antibodies in prophylaxis and treatment of inhalation anthrax.Future Microbiol.2009 Feb;4(1):35-43.
2 How many drug targets are there Nat Rev Drug Discov. 2006 Dec;5(12):993-6.
3 Novel small-molecule inhibitors of anthrax lethal factor identified by high-throughput screening. J Med Chem. 2006 Aug 24;49(17):5232-44.
4 Amiodarone and bepridil inhibit anthrax toxin entry into host cells. Antimicrob Agents Chemother. 2007 Jul;51(7):2403-11.
5 Anthrax lethal factor protease inhibitors: synthesis, SAR, and structure-based 3D QSAR studies. J Med Chem. 2006 Jan 12;49(1):27-30.
6 The discovery of a potent and selective lethal factor inhibitor for adjunct therapy of anthrax infection. Bioorg Med Chem Lett. 2006 Feb 15;16(4):964-8.
7 Inhibitors of anthrax lethal factor. Bioorg Med Chem Lett. 2007 Aug 15;17(16):4575-8.
8 Inhibitors of anthrax lethal factor based upon N-oleoyldopamine. Bioorg Med Chem Lett. 2008 Apr 1;18(7):2467-70.