| DOT Name |
Histone-lysine N-methyltransferase PRDM9 (PRDM9)
|
| Synonyms |
PR domain zinc finger protein 9; PR domain-containing protein 9; Protein-lysine N-methyltransferase PRDM9; EC 2.1.1.-; -lysine36 N-trimethyltransferase PRDM9; EC 2.1.1.359; -lysine4 N-trimethyltransferase PRDM9; EC 2.1.1.354; -lysine9 N-trimethyltransferase PRDM9; EC 2.1.1.355; -N-methyl-L-lysine20 N-methyltransferase PRDM9; EC 2.1.1.362; -lysine20 N-methyltransferase PRDM9; EC 2.1.1.361
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| Gene Name |
PRDM9
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| Related Disease |
- Azoospermia ( )
- Childhood acute lymphoblastic leukemia ( )
- Clear cell renal carcinoma ( )
- DiGeorge syndrome ( )
- Head-neck squamous cell carcinoma ( )
- Male infertility ( )
- Neoplasm ( )
- Oligospermia ( )
- Prader-Willi syndrome ( )
- Shprintzen-Goldberg syndrome ( )
- Velocardiofacial syndrome ( )
- Acute lymphocytic leukaemia ( )
- Advanced cancer ( )
- Cryptorchidism ( )
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| UniProt ID |
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| 3D Structure |
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| PDB ID |
4IJD ; 5EGB ; 5EH2 ; 5EI9 ; 6NM4 |
| EC Number |
2.1.1.-; 2.1.1.354; 2.1.1.355; 2.1.1.359; 2.1.1.361; 2.1.1.362
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| Pfam ID |
PF01352
; PF21549
; PF09514
; PF00096
; PF21225
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| Sequence |
MSPEKSQEESPEEDTERTERKPMVKDAFKDISIYFTKEEWAEMGDWEKTRYRNVKRNYNA
LITIGLRATRPAFMCHRRQAIKLQVDDTEDSDEEWTPRQQVKPPWMALRVEQRKHQKGMP
KASFSNESSLKELSRTANLLNASGSEQAQKPVSPSGEASTSGQHSRLKLELRKKETERKM
YSLRERKGHAYKEVSEPQDDDYLYCEMCQNFFIDSCAAHGPPTFVKDSAVDKGHPNRSAL
SLPPGLRIGPSGIPQAGLGVWNEASDLPLGLHFGPYEGRITEDEEAANNGYSWLITKGRN
CYEYVDGKDKSWANWMRYVNCARDDEEQNLVAFQYHRQIFYRTCRVIRPGCELLVWYGDE
YGQELGIKWGSKWKKELMAGREPKPEIHPCPSCCLAFSSQKFLSQHVERNHSSQNFPGPS
ARKLLQPENPCPGDQNQEQQYPDPHSRNDKTKGQEIKERSKLLNKRTWQREISRAFSSPP
KGQMGSCRVGKRIMEEESRTGQKVNPGNTGKLFVGVGISRIAKVKYGECGQGFSVKSDVI
THQRTHTGEKLYVCRECGRGFSWKSHLLIHQRIHTGEKPYVCRECGRGFSWQSVLLTHQR
THTGEKPYVCRECGRGFSRQSVLLTHQRRHTGEKPYVCRECGRGFSRQSVLLTHQRRHTG
EKPYVCRECGRGFSWQSVLLTHQRTHTGEKPYVCRECGRGFSWQSVLLTHQRTHTGEKPY
VCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRGFRDKSHLLRHQRTHTGEKPYVCRE
CGRGFRDKSNLLSHQRTHTGEKPYVCRECGRGFSNKSHLLRHQRTHTGEKPYVCRECGRG
FRNKSHLLRHQRTHTGEKPYVCRECGRGFSDRSSLCYHQRTHTGEKPYVCREDE
|
| Function |
Histone methyltransferase that sequentially mono-, di-, and tri-methylates both 'Lys-4' (H3K4) and 'Lys-36' (H3K36) of histone H3 to produce respectively trimethylated 'Lys-4' (H3K4me3) and trimethylated 'Lys-36' (H3K36me3) histone H3 and plays a key role in meiotic prophase by determining hotspot localization thereby promoting meiotic recombination. Can also methylate all four core histones with H3 being the best substrate and the most highly modified. Is also able, on one hand, to mono and di-methylate H4K20 and on other hand to trimethylate H3K9 with the di-methylated H3K9 as the best substrate. During meiotic prophase, binds specific DNA sequences through its zinc finger domains thereby determining hotspot localization where it promotes local H3K4me3 and H3K36me3 enrichment on the same nucleosomes through its histone methyltransferase activity. Thereby promotes double-stranded breaks (DSB) formation, at this subset of PRDM9-binding sites, that initiates meiotic recombination for the proper meiotic progression. During meiotic progression hotspot-bound PRDM9 interacts with several complexes; in early leptonema binds CDYL and EHMT2 followed by EWSR1 and CXXC1 by the end of leptonema. EWSR1 joins PRDM9 with the chromosomal axis through REC8. In this way, controls the DSB repair pathway, pairing of homologous chromosomes and sex body formation. Moreover plays a central role in the transcriptional activation of genes during early meiotic prophase thanks to H3K4me3 and H3K36me3 enrichment that represents a specific tag for epigenetic transcriptional activation. In addition performs automethylation. Acetylation and phosphorylation of histone H3 attenuate or prevent histone H3 methylation.
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| KEGG Pathway |
- Lysine degradation (hsa00310 )
- Metabolic pathways (hsa01100 )
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| Reactome Pathway |
- Meiotic recombination (R-HSA-912446 )
- PKMTs methylate histone lysines (R-HSA-3214841 )
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| BioCyc Pathway |
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- MetaCyc:HS09047-MONOMER
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