General Information of Drug Off-Target (DOT) (ID: OTSMGKXQ)

DOT Name 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP)
Synonyms EC 5.3.3.5; Cholestenol Delta-isomerase; Delta(8)-Delta(7) sterol isomerase; D8-D7 sterol isomerase; Emopamil-binding protein
Gene Name EBP
Related Disease
MEND syndrome ( )
Obsolete chondrodysplasia punctata 2, X-linked dominant ( )
X-linked chondrodysplasia punctata 2 ( )
UniProt ID
EBP_HUMAN
3D Structure
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2D Sequence (FASTA)
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3D Structure (PDB)
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PDB ID
6OHT; 6OHU
EC Number
5.3.3.5
Pfam ID
PF05241
Sequence
MTTNAGPLHPYWPQHLRLDNFVPNDRPTWHILAGLFSVTGVLVVTTWLLSGRAAVVPLGT
WRRLSLCWFAVCGFIHLVIEGWFVLYYEDLLGDQAFLSQLWKEYAKGDSRYILGDNFTVC
METITACLWGPLSLWVVIAFLRQHPLRFILQLVVSVGQIYGDVLYFLTEHRDGFQHGELG
HPLYFWFYFVFMNALWLVLPGVLVLDAVKHLTHAQSTLDAKATKAKSKKN
Function Catalyzes the conversion of Delta(8)-sterols to their corresponding Delta(7)-isomers.
KEGG Pathway
Steroid biosynthesis (hsa00100 )
Metabolic pathways (hsa01100 )
Reactome Pathway
Cholesterol biosynthesis via lathosterol (R-HSA-6807062 )
Cholesterol biosynthesis via desmosterol (R-HSA-6807047 )
BioCyc Pathway
MetaCyc:ENSG00000147155-MONOMER

Molecular Interaction Atlas (MIA) of This DOT

3 Disease(s) Related to This DOT
Disease Name Disease ID Evidence Level Mode of Inheritance REF
MEND syndrome DISFRNO5 Definitive X-linked [1]
Obsolete chondrodysplasia punctata 2, X-linked dominant DIS34HL9 Definitive X-linked [2]
X-linked chondrodysplasia punctata 2 DISI20G2 Definitive X-linked [3]
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Molecular Interaction Atlas (MIA) Jump to Detail Molecular Interaction Atlas of This DOT
This DOT Affected the Drug Response of 1 Drug(s)
Drug Name Drug ID Highest Status Interaction REF
Fluorouracil DMUM7HZ Approved 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP) affects the response to substance of Fluorouracil. [29]
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26 Drug(s) Affected the Gene/Protein Processing of This DOT
Drug Name Drug ID Highest Status Interaction REF
Valproate DMCFE9I Approved Valproate decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [4]
Ciclosporin DMAZJFX Approved Ciclosporin decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [5]
Tretinoin DM49DUI Approved Tretinoin increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [6]
Acetaminophen DMUIE76 Approved Acetaminophen decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [7]
Doxorubicin DMVP5YE Approved Doxorubicin increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [8]
Cupric Sulfate DMP0NFQ Approved Cupric Sulfate decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [9]
Cisplatin DMRHGI9 Approved Cisplatin increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [10]
Estradiol DMUNTE3 Approved Estradiol decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [5]
Ivermectin DMDBX5F Approved Ivermectin decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [11]
Temozolomide DMKECZD Approved Temozolomide decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [12]
Carbamazepine DMZOLBI Approved Carbamazepine affects the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [13]
Marinol DM70IK5 Approved Marinol decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [14]
Zoledronate DMIXC7G Approved Zoledronate increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [15]
Progesterone DMUY35B Approved Progesterone increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [16]
Isotretinoin DM4QTBN Approved Isotretinoin decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [17]
Piroxicam DMTK234 Approved Piroxicam decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [18]
Bicalutamide DMZMSPF Approved Bicalutamide decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [19]
Fluoxetine DM3PD2C Approved Fluoxetine increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [20]
Genistein DM0JETC Phase 2/3 Genistein increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [21]
PEITC DMOMN31 Phase 2 PEITC decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [22]
PMID28460551-Compound-2 DM4DOUB Patented PMID28460551-Compound-2 increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [24]
Bisphenol A DM2ZLD7 Investigative Bisphenol A increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [21]
Formaldehyde DM7Q6M0 Investigative Formaldehyde decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [25]
Coumestrol DM40TBU Investigative Coumestrol increases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [26]
Arachidonic acid DMUOQZD Investigative Arachidonic acid decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [27]
PP-242 DM2348V Investigative PP-242 decreases the expression of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [28]
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⏷ Show the Full List of 26 Drug(s)
1 Drug(s) Affected the Post-Translational Modifications of This DOT
Drug Name Drug ID Highest Status Interaction REF
Benzo(a)pyrene DMN7J43 Phase 1 Benzo(a)pyrene decreases the methylation of 3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase (EBP). [23]
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References

1 Technical standards for the interpretation and reporting of constitutional copy-number variants: a joint consensus recommendation of the American College of Medical Genetics and Genomics (ACMG) and the Clinical Genome Resource (ClinGen). Genet Med. 2020 Feb;22(2):245-257. doi: 10.1038/s41436-019-0686-8. Epub 2019 Nov 6.
2 Clinical, molecular and biochemical characterization of nine Spanish families with Conradi-Hnermann-Happle syndrome: new insights into X-linked dominant chondrodysplasia punctata with a comprehensive review of the literature. Br J Dermatol. 2012 Apr;166(4):830-8. doi: 10.1111/j.1365-2133.2011.10756.x. Epub 2012 Mar 2.
3 Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hnermann syndrome. Nat Genet. 1999 Jul;22(3):291-4. doi: 10.1038/10357.
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9 Physiological and toxicological transcriptome changes in HepG2 cells exposed to copper. Physiol Genomics. 2009 Aug 7;38(3):386-401.
10 Low doses of cisplatin induce gene alterations, cell cycle arrest, and apoptosis in human promyelocytic leukemia cells. Biomark Insights. 2016 Aug 24;11:113-21.
11 Quantitative proteomics reveals a broad-spectrum antiviral property of ivermectin, benefiting for COVID-19 treatment. J Cell Physiol. 2021 Apr;236(4):2959-2975. doi: 10.1002/jcp.30055. Epub 2020 Sep 22.
12 Temozolomide induces activation of Wnt/-catenin signaling in glioma cells via PI3K/Akt pathway: implications in glioma therapy. Cell Biol Toxicol. 2020 Jun;36(3):273-278. doi: 10.1007/s10565-019-09502-7. Epub 2019 Nov 22.
13 Gene Expression Regulation and Pathway Analysis After Valproic Acid and Carbamazepine Exposure in a Human Embryonic Stem Cell-Based Neurodevelopmental Toxicity Assay. Toxicol Sci. 2015 Aug;146(2):311-20. doi: 10.1093/toxsci/kfv094. Epub 2015 May 15.
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16 Coordinate up-regulation of TMEM97 and cholesterol biosynthesis genes in normal ovarian surface epithelial cells treated with progesterone: implications for pathogenesis of ovarian cancer. BMC Cancer. 2007 Dec 11;7:223.
17 Temporal changes in gene expression in the skin of patients treated with isotretinoin provide insight into its mechanism of action. Dermatoendocrinol. 2009 May;1(3):177-87.
18 Apoptosis induced by piroxicam plus cisplatin combined treatment is triggered by p21 in mesothelioma. PLoS One. 2011;6(8):e23569.
19 Microarray analysis of bicalutamide action on telomerase activity, p53 pathway and viability of prostate carcinoma cell lines. J Pharm Pharmacol. 2005 Jan;57(1):83-92.
20 Screening autism-associated environmental factors in differentiating human neural progenitors with fractional factorial design-based transcriptomics. Sci Rep. 2023 Jun 29;13(1):10519. doi: 10.1038/s41598-023-37488-0.
21 Convergent transcriptional profiles induced by endogenous estrogen and distinct xenoestrogens in breast cancer cells. Carcinogenesis. 2006 Aug;27(8):1567-78.
22 Phenethyl isothiocyanate alters the gene expression and the levels of protein associated with cell cycle regulation in human glioblastoma GBM 8401 cells. Environ Toxicol. 2017 Jan;32(1):176-187.
23 Air pollution and DNA methylation alterations in lung cancer: A systematic and comparative study. Oncotarget. 2017 Jan 3;8(1):1369-1391. doi: 10.18632/oncotarget.13622.
24 Cell-based two-dimensional morphological assessment system to predict cancer drug-induced cardiotoxicity using human induced pluripotent stem cell-derived cardiomyocytes. Toxicol Appl Pharmacol. 2019 Nov 15;383:114761. doi: 10.1016/j.taap.2019.114761. Epub 2019 Sep 15.
25 Characterization of formaldehyde's genotoxic mode of action by gene expression analysis in TK6 cells. Arch Toxicol. 2013 Nov;87(11):1999-2012.
26 Pleiotropic combinatorial transcriptomes of human breast cancer cells exposed to mixtures of dietary phytoestrogens. Food Chem Toxicol. 2009 Apr;47(4):787-95.
27 Arachidonic acid-induced gene expression in colon cancer cells. Carcinogenesis. 2006 Oct;27(10):1950-60.
28 Marine biogenics in sea spray aerosols interact with the mTOR signaling pathway. Sci Rep. 2019 Jan 24;9(1):675.
29 Mechanistic and predictive profiling of 5-Fluorouracil resistance in human cancer cells. Cancer Res. 2004 Nov 15;64(22):8167-76. doi: 10.1158/0008-5472.CAN-04-0970.